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Actin Filaments That Form Networks in Living Cells Fluctuate Rapidly and Independently of Each Other

Actin filaments play a significant role in multiple essential cellular processes, including cell motility, vesicle and organelle movement, cell signaling, and cellular mechanosensing mechanisms. However, an important cellular processes, mechanosensing, remains debatable. This is because intracellular proteins such as actin filaments, focal adhesion complexes, and cell-nuclear junctions are dynamic structures that fluctuate minutely, although their binding is closely related to the mechanosensing mechanism. We established an original quasi-super-resolution image analysis method and revealed the existence of 3 Hz fluctuations in actin filaments in living cells at approximately 0.2 to 0.5 μm. We speculated that cells sense mechanical stresses such as fluid shear stress through the network structure of actin filaments and their connections to the substrate and cell nucleus. This study analyzed the fluctuations in actin filaments in the network structure of living cells using our quasi-super-resolution image analysis method under static culture conditions. In particular, we focused on the correlations between each actin fluctuation in the network structure. Fluorescence images showed that actin networks were well developed in the NIH3T3 cells. The maximum amplitude of actin filament fluctuations near the central region of the cell was 0.99 μm. Correlation coefficients of actin filament fluctuations in the network remained unchanged between the central and peripheral regions, with a maximum value of 0.23. These results suggested that actin filaments fluctuated independently within the network structure. Moreover, the distance between two actin filaments changed over time at the connecting point of the three actin filaments. These results suggest that strain occurs at the actin filament connecting points even when cells are under static culture conditions and that more complex mechanical states arise upon mechanical stimulation.

Actin Filament, Fluctuation, Lifeact-GFP, Living Cell, Network Structure, Super-Resolution Technique

APA Style

Tomoteru Oka, Kouki Furukawa, Yasuyuki Oguma, Buntara Sthenly Gan, Noriyuki Kataoka. (2023). Actin Filaments That Form Networks in Living Cells Fluctuate Rapidly and Independently of Each Other. International Journal of Biomedical Science and Engineering, 11(3), 33-43. https://doi.org/10.11648/j.ijbse.20231103.11

ACS Style

Tomoteru Oka; Kouki Furukawa; Yasuyuki Oguma; Buntara Sthenly Gan; Noriyuki Kataoka. Actin Filaments That Form Networks in Living Cells Fluctuate Rapidly and Independently of Each Other. Int. J. Biomed. Sci. Eng. 2023, 11(3), 33-43. doi: 10.11648/j.ijbse.20231103.11

AMA Style

Tomoteru Oka, Kouki Furukawa, Yasuyuki Oguma, Buntara Sthenly Gan, Noriyuki Kataoka. Actin Filaments That Form Networks in Living Cells Fluctuate Rapidly and Independently of Each Other. Int J Biomed Sci Eng. 2023;11(3):33-43. doi: 10.11648/j.ijbse.20231103.11

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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