Immunohistochemical and Stereological Studies of <i>Treculia africana</i> Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (<i>Rattus novergicus</i>)

Obasi Kosisochukwu Kingsley; Lambe Ezra; Oyewopo Adetunji; Anyanwu Emeka

doi:doi:10.11648/j.ijbse.20221001.12

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Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus)

Obasi Kosisochukwu Kingsley, Lambe Ezra, Oyewopo Adetunji, Anyanwu Emeka

Published in International Journal of Biomedical Science and Engineering (Volume 10, Issue 1)

Received: 7 April 2021 Accepted: 24 April 2021 Published: 24 January 2022

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Abstract

Introduction: Chloroquine causes damage to the testis by crossing the blood testis barrier. Treculia africana has numerous medicinal potentials. However, the prevention of its complication such as male infertility remains unexplored. This aim of this study was to evaluate the effects of aqueous seed extract of Treculia africana on chloroquine-induced toxicity in the testis of adult male Wistar rats (Rattus norvegicus). Methods: Twenty eight (28) rats with an average weight 200±10g were used for the study and divided into four groups consisting of seven rats in each group. Control group A received 1 ml of distilled water daily, group B received 2mg/kg body weight daily of chloroquine orally, group C received 200mg/kg body weight daily of aqueous seed extract of Treculia Africana while group D received 2mg/kg body weight daily of chloroquine and Treculia africana seed extract (TASE) concurrently for a period of 60 days. Histological, histopathological, hormonal and semen analysis, Stereological and Imunohistochemical evaluation of the testis were done. Results: Histological analysis showed the ameliorative properties of TASE after chloroquine induced testicular toxicity. Significant differences (P<0.05) in hormonal analysis were observed across treatment groups. Significant differences (P<0.05) in Semen and Sterological analysis were observed. Johnsen’s Score of the testis showed a significant improvement in group D while Immunohistochemical observation showed the ameliorative properties of TASE. Conclusion: chloroquine is injurious to male reproductive health, administration of TASE may help to improve seminiferous tubule integrity and immunohistochemistry and stereological studies further revealed its ameliorative properties.

Published in	International Journal of Biomedical Science and Engineering (Volume 10, Issue 1)
DOI	10.11648/j.ijbse.20221001.12
Page(s)	12-18
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Treculia africana Seed Extract (TASE), Chloroquine, Testis, Immunohistochemical, Stereology

References

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APA Style

Obasi Kosisochukwu Kingsley, Lambe Ezra, Oyewopo Adetunji, Anyanwu Emeka. (2022). Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus). International Journal of Biomedical Science and Engineering, 10(1), 12-18. https://doi.org/10.11648/j.ijbse.20221001.12

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ACS Style

Obasi Kosisochukwu Kingsley; Lambe Ezra; Oyewopo Adetunji; Anyanwu Emeka. Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus). Int. J. Biomed. Sci. Eng. 2022, 10(1), 12-18. doi: 10.11648/j.ijbse.20221001.12

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AMA Style

Obasi Kosisochukwu Kingsley, Lambe Ezra, Oyewopo Adetunji, Anyanwu Emeka. Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus). Int J Biomed Sci Eng. 2022;10(1):12-18. doi: 10.11648/j.ijbse.20221001.12

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@article{10.11648/j.ijbse.20221001.12,
  author = {Obasi Kosisochukwu Kingsley and Lambe Ezra and Oyewopo Adetunji and Anyanwu Emeka},
  title = {Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus)},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {10},
  number = {1},
  pages = {12-18},
  doi = {10.11648/j.ijbse.20221001.12},
  url = {https://doi.org/10.11648/j.ijbse.20221001.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20221001.12},
  abstract = {Introduction: Chloroquine causes damage to the testis by crossing the blood testis barrier. Treculia africana has numerous medicinal potentials. However, the prevention of its complication such as male infertility remains unexplored. This aim of this study was to evaluate the effects of aqueous seed extract of Treculia africana on chloroquine-induced toxicity in the testis of adult male Wistar rats (Rattus norvegicus). Methods: Twenty eight (28) rats with an average weight 200±10g were used for the study and divided into four groups consisting of seven rats in each group. Control group A received 1 ml of distilled water daily, group B received 2mg/kg body weight daily of chloroquine orally, group C received 200mg/kg body weight daily of aqueous seed extract of Treculia Africana while group D received 2mg/kg body weight daily of chloroquine and Treculia africana seed extract (TASE) concurrently for a period of 60 days. Histological, histopathological, hormonal and semen analysis, Stereological and Imunohistochemical evaluation of the testis were done. Results: Histological analysis showed the ameliorative properties of TASE after chloroquine induced testicular toxicity. Significant differences (PConclusion: chloroquine is injurious to male reproductive health, administration of TASE may help to improve seminiferous tubule integrity and immunohistochemistry and stereological studies further revealed its ameliorative properties.},
 year = {2022}
}

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TY  - JOUR
T1  - Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus)
AU  - Obasi Kosisochukwu Kingsley
AU  - Lambe Ezra
AU  - Oyewopo Adetunji
AU  - Anyanwu Emeka
Y1  - 2022/01/24
PY  - 2022
N1  - https://doi.org/10.11648/j.ijbse.20221001.12
DO  - 10.11648/j.ijbse.20221001.12
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 12
EP  - 18
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20221001.12
AB  - Introduction: Chloroquine causes damage to the testis by crossing the blood testis barrier. Treculia africana has numerous medicinal potentials. However, the prevention of its complication such as male infertility remains unexplored. This aim of this study was to evaluate the effects of aqueous seed extract of Treculia africana on chloroquine-induced toxicity in the testis of adult male Wistar rats (Rattus norvegicus). Methods: Twenty eight (28) rats with an average weight 200±10g were used for the study and divided into four groups consisting of seven rats in each group. Control group A received 1 ml of distilled water daily, group B received 2mg/kg body weight daily of chloroquine orally, group C received 200mg/kg body weight daily of aqueous seed extract of Treculia Africana while group D received 2mg/kg body weight daily of chloroquine and Treculia africana seed extract (TASE) concurrently for a period of 60 days. Histological, histopathological, hormonal and semen analysis, Stereological and Imunohistochemical evaluation of the testis were done. Results: Histological analysis showed the ameliorative properties of TASE after chloroquine induced testicular toxicity. Significant differences (PConclusion: chloroquine is injurious to male reproductive health, administration of TASE may help to improve seminiferous tubule integrity and immunohistochemistry and stereological studies further revealed its ameliorative properties.
VL  - 10
IS  - 1
ER  -

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Author Information

Obasi Kosisochukwu Kingsley

Department of Anatomy, University of Nigeria Enugu Campus, Enugu, Nigeria
Lambe Ezra

Department of Anatomy, University of Ilorin, Ilorin, Nigeria
Oyewopo Adetunji

Department of Anatomy, University of Ilorin, Ilorin, Nigeria
Anyanwu Emeka

Department of Anatomy, University of Nigeria Enugu Campus, Enugu, Nigeria

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APA Style

Obasi Kosisochukwu Kingsley, Lambe Ezra, Oyewopo Adetunji, Anyanwu Emeka. (2022). Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus). International Journal of Biomedical Science and Engineering, 10(1), 12-18. https://doi.org/10.11648/j.ijbse.20221001.12

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ACS Style

Obasi Kosisochukwu Kingsley; Lambe Ezra; Oyewopo Adetunji; Anyanwu Emeka. Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus). Int. J. Biomed. Sci. Eng. 2022, 10(1), 12-18. doi: 10.11648/j.ijbse.20221001.12

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AMA Style

Obasi Kosisochukwu Kingsley, Lambe Ezra, Oyewopo Adetunji, Anyanwu Emeka. Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus). Int J Biomed Sci Eng. 2022;10(1):12-18. doi: 10.11648/j.ijbse.20221001.12

Copy | Download

@article{10.11648/j.ijbse.20221001.12,
  author = {Obasi Kosisochukwu Kingsley and Lambe Ezra and Oyewopo Adetunji and Anyanwu Emeka},
  title = {Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus)},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {10},
  number = {1},
  pages = {12-18},
  doi = {10.11648/j.ijbse.20221001.12},
  url = {https://doi.org/10.11648/j.ijbse.20221001.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20221001.12},
  abstract = {Introduction: Chloroquine causes damage to the testis by crossing the blood testis barrier. Treculia africana has numerous medicinal potentials. However, the prevention of its complication such as male infertility remains unexplored. This aim of this study was to evaluate the effects of aqueous seed extract of Treculia africana on chloroquine-induced toxicity in the testis of adult male Wistar rats (Rattus norvegicus). Methods: Twenty eight (28) rats with an average weight 200±10g were used for the study and divided into four groups consisting of seven rats in each group. Control group A received 1 ml of distilled water daily, group B received 2mg/kg body weight daily of chloroquine orally, group C received 200mg/kg body weight daily of aqueous seed extract of Treculia Africana while group D received 2mg/kg body weight daily of chloroquine and Treculia africana seed extract (TASE) concurrently for a period of 60 days. Histological, histopathological, hormonal and semen analysis, Stereological and Imunohistochemical evaluation of the testis were done. Results: Histological analysis showed the ameliorative properties of TASE after chloroquine induced testicular toxicity. Significant differences (PConclusion: chloroquine is injurious to male reproductive health, administration of TASE may help to improve seminiferous tubule integrity and immunohistochemistry and stereological studies further revealed its ameliorative properties.},
 year = {2022}
}

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TY  - JOUR
T1  - Immunohistochemical and Stereological Studies of Treculia africana Seed on Chloroquine Induced Damage on the Testes of Adult Male Wistar Rats (Rattus novergicus)
AU  - Obasi Kosisochukwu Kingsley
AU  - Lambe Ezra
AU  - Oyewopo Adetunji
AU  - Anyanwu Emeka
Y1  - 2022/01/24
PY  - 2022
N1  - https://doi.org/10.11648/j.ijbse.20221001.12
DO  - 10.11648/j.ijbse.20221001.12
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 12
EP  - 18
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20221001.12
AB  - Introduction: Chloroquine causes damage to the testis by crossing the blood testis barrier. Treculia africana has numerous medicinal potentials. However, the prevention of its complication such as male infertility remains unexplored. This aim of this study was to evaluate the effects of aqueous seed extract of Treculia africana on chloroquine-induced toxicity in the testis of adult male Wistar rats (Rattus norvegicus). Methods: Twenty eight (28) rats with an average weight 200±10g were used for the study and divided into four groups consisting of seven rats in each group. Control group A received 1 ml of distilled water daily, group B received 2mg/kg body weight daily of chloroquine orally, group C received 200mg/kg body weight daily of aqueous seed extract of Treculia Africana while group D received 2mg/kg body weight daily of chloroquine and Treculia africana seed extract (TASE) concurrently for a period of 60 days. Histological, histopathological, hormonal and semen analysis, Stereological and Imunohistochemical evaluation of the testis were done. Results: Histological analysis showed the ameliorative properties of TASE after chloroquine induced testicular toxicity. Significant differences (PConclusion: chloroquine is injurious to male reproductive health, administration of TASE may help to improve seminiferous tubule integrity and immunohistochemistry and stereological studies further revealed its ameliorative properties.
VL  - 10
IS  - 1
ER  -

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