An In-vitro Method for Current Induced Ventricular Fibrillations

Michael Koch; Michael Bartonek; Jiri Silny; David Santer; Bruno Podesser

doi:doi:10.11648/j.ijbse.20221001.11

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An In-vitro Method for Current Induced Ventricular Fibrillations

Michael Koch, Michael Bartonek, Jiri Silny, David Santer, Bruno Podesser

Published in International Journal of Biomedical Science and Engineering (Volume 10, Issue 1)

Received: 29 November 2021 Accepted: 10 January 2022 Published: 18 January 2022

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Abstract

The motivation of the current study was to provide threshold values of rabbit hearts for ventricular fibrillation (vf) induced by electric current of 50Hz and to develop a flexible but robust experimental setup for stimulation experiments on the beating heart. The stimulation was performed in a way that the current flow through the heart is nearly homogeneous, similar to the situation of an electrical accident. In this way the work should also serve as a basis for future vf-related investigations, e.g. sine waves from 10Hz up to 10kHz, mixed signals, current pulses and pure DC. One of the main objectives was to avoid deviations of the data due to inappropriate experimental methods, such like direct contact of the heart tissue or interferences from the nervous system which one get from whole animal experiments. The work should additionally give the proper setup to gather the necessary data for transformations of animal data to human data. First an experimental procedure and setup for hearts of small animals that fulfills most important requirements for stimulation of the myocardium and the measurement of various heart parameters in a repeatable way was developed. In this way specific stimulation experiments were performed on rabbit hearts in an ejecting, blood-perfused isolated heart model to determine the threshold values for vf at 50Hz. Additional experiments to determine the electrical field inside and outside of the heart as well as the dependency of different stimuli modes (T-wave trigger, stimulation for several periods) have been conducted. In the verification with a frequency of 50Hz, current density of (7,3 ± 3,8)mA/cm² results as a mean threshold of ventricular fibrillation from 143 experiments. Finally a comparison to other research works in this field was performed to show the advantages and disadvantages of the respective approaches.

Published in	International Journal of Biomedical Science and Engineering (Volume 10, Issue 1)
DOI	10.11648/j.ijbse.20221001.11
Page(s)	1-11
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ventricular Fibrillation, Isolated Heart Model, Vf-threshold, Rabbit Hearts, Experimental Setup

References

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APA Style

Michael Koch, Michael Bartonek, Jiri Silny, David Santer, Bruno Podesser. (2022). An In-vitro Method for Current Induced Ventricular Fibrillations. International Journal of Biomedical Science and Engineering, 10(1), 1-11. https://doi.org/10.11648/j.ijbse.20221001.11

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ACS Style

Michael Koch; Michael Bartonek; Jiri Silny; David Santer; Bruno Podesser. An In-vitro Method for Current Induced Ventricular Fibrillations. Int. J. Biomed. Sci. Eng. 2022, 10(1), 1-11. doi: 10.11648/j.ijbse.20221001.11

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AMA Style

Michael Koch, Michael Bartonek, Jiri Silny, David Santer, Bruno Podesser. An In-vitro Method for Current Induced Ventricular Fibrillations. Int J Biomed Sci Eng. 2022;10(1):1-11. doi: 10.11648/j.ijbse.20221001.11

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@article{10.11648/j.ijbse.20221001.11,
  author = {Michael Koch and Michael Bartonek and Jiri Silny and David Santer and Bruno Podesser},
  title = {An In-vitro Method for Current Induced Ventricular Fibrillations},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {10},
  number = {1},
  pages = {1-11},
  doi = {10.11648/j.ijbse.20221001.11},
  url = {https://doi.org/10.11648/j.ijbse.20221001.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20221001.11},
  abstract = {The motivation of the current study was to provide threshold values of rabbit hearts for ventricular fibrillation (vf) induced by electric current of 50Hz and to develop a flexible but robust experimental setup for stimulation experiments on the beating heart. The stimulation was performed in a way that the current flow through the heart is nearly homogeneous, similar to the situation of an electrical accident. In this way the work should also serve as a basis for future vf-related investigations, e.g. sine waves from 10Hz up to 10kHz, mixed signals, current pulses and pure DC. One of the main objectives was to avoid deviations of the data due to inappropriate experimental methods, such like direct contact of the heart tissue or interferences from the nervous system which one get from whole animal experiments. The work should additionally give the proper setup to gather the necessary data for transformations of animal data to human data. First an experimental procedure and setup for hearts of small animals that fulfills most important requirements for stimulation of the myocardium and the measurement of various heart parameters in a repeatable way was developed. In this way specific stimulation experiments were performed on rabbit hearts in an ejecting, blood-perfused isolated heart model to determine the threshold values for vf at 50Hz. Additional experiments to determine the electrical field inside and outside of the heart as well as the dependency of different stimuli modes (T-wave trigger, stimulation for several periods) have been conducted. In the verification with a frequency of 50Hz, current density of (7,3 ± 3,8)mA/cm2 results as a mean threshold of ventricular fibrillation from 143 experiments. Finally a comparison to other research works in this field was performed to show the advantages and disadvantages of the respective approaches.},
 year = {2022}
}

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TY  - JOUR
T1  - An In-vitro Method for Current Induced Ventricular Fibrillations
AU  - Michael Koch
AU  - Michael Bartonek
AU  - Jiri Silny
AU  - David Santer
AU  - Bruno Podesser
Y1  - 2022/01/18
PY  - 2022
N1  - https://doi.org/10.11648/j.ijbse.20221001.11
DO  - 10.11648/j.ijbse.20221001.11
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 1
EP  - 11
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20221001.11
AB  - The motivation of the current study was to provide threshold values of rabbit hearts for ventricular fibrillation (vf) induced by electric current of 50Hz and to develop a flexible but robust experimental setup for stimulation experiments on the beating heart. The stimulation was performed in a way that the current flow through the heart is nearly homogeneous, similar to the situation of an electrical accident. In this way the work should also serve as a basis for future vf-related investigations, e.g. sine waves from 10Hz up to 10kHz, mixed signals, current pulses and pure DC. One of the main objectives was to avoid deviations of the data due to inappropriate experimental methods, such like direct contact of the heart tissue or interferences from the nervous system which one get from whole animal experiments. The work should additionally give the proper setup to gather the necessary data for transformations of animal data to human data. First an experimental procedure and setup for hearts of small animals that fulfills most important requirements for stimulation of the myocardium and the measurement of various heart parameters in a repeatable way was developed. In this way specific stimulation experiments were performed on rabbit hearts in an ejecting, blood-perfused isolated heart model to determine the threshold values for vf at 50Hz. Additional experiments to determine the electrical field inside and outside of the heart as well as the dependency of different stimuli modes (T-wave trigger, stimulation for several periods) have been conducted. In the verification with a frequency of 50Hz, current density of (7,3 ± 3,8)mA/cm2 results as a mean threshold of ventricular fibrillation from 143 experiments. Finally a comparison to other research works in this field was performed to show the advantages and disadvantages of the respective approaches.
VL  - 10
IS  - 1
ER  -

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Author Information

Michael Koch

Eaton Industries, Vienna, Austria
Michael Bartonek

Eaton Industries, Vienna, Austria
Jiri Silny

Femu, Rheinisch-Westfä
David Santer

Ludwig Boltzmann Institute for Cardiovascular Research, Ludwig Boltzmann Gesellschaft, Vienna, Austria
Bruno Podesser

Ludwig Boltzmann Institute for Cardiovascular Research, Ludwig Boltzmann Gesellschaft, Vienna, Austria

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APA Style

Michael Koch, Michael Bartonek, Jiri Silny, David Santer, Bruno Podesser. (2022). An In-vitro Method for Current Induced Ventricular Fibrillations. International Journal of Biomedical Science and Engineering, 10(1), 1-11. https://doi.org/10.11648/j.ijbse.20221001.11

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ACS Style

Michael Koch; Michael Bartonek; Jiri Silny; David Santer; Bruno Podesser. An In-vitro Method for Current Induced Ventricular Fibrillations. Int. J. Biomed. Sci. Eng. 2022, 10(1), 1-11. doi: 10.11648/j.ijbse.20221001.11

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AMA Style

Michael Koch, Michael Bartonek, Jiri Silny, David Santer, Bruno Podesser. An In-vitro Method for Current Induced Ventricular Fibrillations. Int J Biomed Sci Eng. 2022;10(1):1-11. doi: 10.11648/j.ijbse.20221001.11

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@article{10.11648/j.ijbse.20221001.11,
  author = {Michael Koch and Michael Bartonek and Jiri Silny and David Santer and Bruno Podesser},
  title = {An In-vitro Method for Current Induced Ventricular Fibrillations},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {10},
  number = {1},
  pages = {1-11},
  doi = {10.11648/j.ijbse.20221001.11},
  url = {https://doi.org/10.11648/j.ijbse.20221001.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20221001.11},
  abstract = {The motivation of the current study was to provide threshold values of rabbit hearts for ventricular fibrillation (vf) induced by electric current of 50Hz and to develop a flexible but robust experimental setup for stimulation experiments on the beating heart. The stimulation was performed in a way that the current flow through the heart is nearly homogeneous, similar to the situation of an electrical accident. In this way the work should also serve as a basis for future vf-related investigations, e.g. sine waves from 10Hz up to 10kHz, mixed signals, current pulses and pure DC. One of the main objectives was to avoid deviations of the data due to inappropriate experimental methods, such like direct contact of the heart tissue or interferences from the nervous system which one get from whole animal experiments. The work should additionally give the proper setup to gather the necessary data for transformations of animal data to human data. First an experimental procedure and setup for hearts of small animals that fulfills most important requirements for stimulation of the myocardium and the measurement of various heart parameters in a repeatable way was developed. In this way specific stimulation experiments were performed on rabbit hearts in an ejecting, blood-perfused isolated heart model to determine the threshold values for vf at 50Hz. Additional experiments to determine the electrical field inside and outside of the heart as well as the dependency of different stimuli modes (T-wave trigger, stimulation for several periods) have been conducted. In the verification with a frequency of 50Hz, current density of (7,3 ± 3,8)mA/cm2 results as a mean threshold of ventricular fibrillation from 143 experiments. Finally a comparison to other research works in this field was performed to show the advantages and disadvantages of the respective approaches.},
 year = {2022}
}

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TY  - JOUR
T1  - An In-vitro Method for Current Induced Ventricular Fibrillations
AU  - Michael Koch
AU  - Michael Bartonek
AU  - Jiri Silny
AU  - David Santer
AU  - Bruno Podesser
Y1  - 2022/01/18
PY  - 2022
N1  - https://doi.org/10.11648/j.ijbse.20221001.11
DO  - 10.11648/j.ijbse.20221001.11
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 1
EP  - 11
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20221001.11
AB  - The motivation of the current study was to provide threshold values of rabbit hearts for ventricular fibrillation (vf) induced by electric current of 50Hz and to develop a flexible but robust experimental setup for stimulation experiments on the beating heart. The stimulation was performed in a way that the current flow through the heart is nearly homogeneous, similar to the situation of an electrical accident. In this way the work should also serve as a basis for future vf-related investigations, e.g. sine waves from 10Hz up to 10kHz, mixed signals, current pulses and pure DC. One of the main objectives was to avoid deviations of the data due to inappropriate experimental methods, such like direct contact of the heart tissue or interferences from the nervous system which one get from whole animal experiments. The work should additionally give the proper setup to gather the necessary data for transformations of animal data to human data. First an experimental procedure and setup for hearts of small animals that fulfills most important requirements for stimulation of the myocardium and the measurement of various heart parameters in a repeatable way was developed. In this way specific stimulation experiments were performed on rabbit hearts in an ejecting, blood-perfused isolated heart model to determine the threshold values for vf at 50Hz. Additional experiments to determine the electrical field inside and outside of the heart as well as the dependency of different stimuli modes (T-wave trigger, stimulation for several periods) have been conducted. In the verification with a frequency of 50Hz, current density of (7,3 ± 3,8)mA/cm2 results as a mean threshold of ventricular fibrillation from 143 experiments. Finally a comparison to other research works in this field was performed to show the advantages and disadvantages of the respective approaches.
VL  - 10
IS  - 1
ER  -

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