Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles

Ting Lei Zhao; Zhe Zhang; Dan Li; Yanxialei Jiang

doi:doi:10.11648/j.ijbse.20221002.14

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Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles

Ting Lei Zhao, Zhe Zhang, Dan Li, Yanxialei Jiang

Published in International Journal of Biomedical Science and Engineering (Volume 10, Issue 2)

Received: 31 May 2022 Accepted: 15 June 2022 Published: 27 June 2022

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Abstract

Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination of physical and optical properties. Sea urchin-like gold nanoparticles (SUL-AuNPs) are well characterized multi-thorn gold nanostructures which possess at least four gold nanothorns on spherical gold surface, mimicking the morphology of sea urchin. Compared to spherical AuNPs, SUL-AuNPs showed a wide variety of light absorption and scattering properties, and the Surface-enhanced Raman Scattering (SERS) properties of SUL-AuNPs were also widely studied dependent on their surface morphology. Herein, three different diameters of SUL-AuNPs based on spherical AuNPs seed-mediated growth method by altering the amount of AuNPs in reaction system had been synthesized. The UV-vis spectrum of synthesized SUL-AuNPs displayed a shift from 550 nm to 650 nm compared to spherical AuNPs. FRET method was applied for the detection of GSH in hepatocytes and cell extracts using rhodamine B (RB) functionalized SUL-AuNPs, among the synthesized three different diameters SUL-AuNPs, 100nm RB-SUL-AuNPs displayed highest sensitivity for GSH detection. What's more, all synthesized SUL-AuNPs turned out to be membrane-permeable, and displayed ignorable cytotoxicity, which make SUL-AuNPs promising cellular thiols detection probes. In particular, it should be noted that the application of RB functionalized SUL-AuNPs exemplify ongoing efforts in design and utility of multifunctional nanoplatforms of SUL-AuNPs.

Published in	International Journal of Biomedical Science and Engineering (Volume 10, Issue 2)
DOI	10.11648/j.ijbse.20221002.14
Page(s)	54-60
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

FRET, Glutathione, Sea Urchin-Like Gold Nanoparticle, Thiol Detection

References

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APA Style

Ting Lei Zhao, Zhe Zhang, Dan Li, Yanxialei Jiang. (2022). Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. International Journal of Biomedical Science and Engineering, 10(2), 54-60. https://doi.org/10.11648/j.ijbse.20221002.14

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ACS Style

Ting Lei Zhao; Zhe Zhang; Dan Li; Yanxialei Jiang. Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. Int. J. Biomed. Sci. Eng. 2022, 10(2), 54-60. doi: 10.11648/j.ijbse.20221002.14

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AMA Style

Ting Lei Zhao, Zhe Zhang, Dan Li, Yanxialei Jiang. Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. Int J Biomed Sci Eng. 2022;10(2):54-60. doi: 10.11648/j.ijbse.20221002.14

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@article{10.11648/j.ijbse.20221002.14,
  author = {Ting Lei Zhao and Zhe Zhang and Dan Li and Yanxialei Jiang},
  title = {Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {10},
  number = {2},
  pages = {54-60},
  doi = {10.11648/j.ijbse.20221002.14},
  url = {https://doi.org/10.11648/j.ijbse.20221002.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20221002.14},
  abstract = {Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination of physical and optical properties. Sea urchin-like gold nanoparticles (SUL-AuNPs) are well characterized multi-thorn gold nanostructures which possess at least four gold nanothorns on spherical gold surface, mimicking the morphology of sea urchin. Compared to spherical AuNPs, SUL-AuNPs showed a wide variety of light absorption and scattering properties, and the Surface-enhanced Raman Scattering (SERS) properties of SUL-AuNPs were also widely studied dependent on their surface morphology. Herein, three different diameters of SUL-AuNPs based on spherical AuNPs seed-mediated growth method by altering the amount of AuNPs in reaction system had been synthesized. The UV-vis spectrum of synthesized SUL-AuNPs displayed a shift from 550 nm to 650 nm compared to spherical AuNPs. FRET method was applied for the detection of GSH in hepatocytes and cell extracts using rhodamine B (RB) functionalized SUL-AuNPs, among the synthesized three different diameters SUL-AuNPs, 100nm RB-SUL-AuNPs displayed highest sensitivity for GSH detection. What's more, all synthesized SUL-AuNPs turned out to be membrane-permeable, and displayed ignorable cytotoxicity, which make SUL-AuNPs promising cellular thiols detection probes. In particular, it should be noted that the application of RB functionalized SUL-AuNPs exemplify ongoing efforts in design and utility of multifunctional nanoplatforms of SUL-AuNPs.},
 year = {2022}
}

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TY  - JOUR
T1  - Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles
AU  - Ting Lei Zhao
AU  - Zhe Zhang
AU  - Dan Li
AU  - Yanxialei Jiang
Y1  - 2022/06/27
PY  - 2022
N1  - https://doi.org/10.11648/j.ijbse.20221002.14
DO  - 10.11648/j.ijbse.20221002.14
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 54
EP  - 60
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20221002.14
AB  - Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination of physical and optical properties. Sea urchin-like gold nanoparticles (SUL-AuNPs) are well characterized multi-thorn gold nanostructures which possess at least four gold nanothorns on spherical gold surface, mimicking the morphology of sea urchin. Compared to spherical AuNPs, SUL-AuNPs showed a wide variety of light absorption and scattering properties, and the Surface-enhanced Raman Scattering (SERS) properties of SUL-AuNPs were also widely studied dependent on their surface morphology. Herein, three different diameters of SUL-AuNPs based on spherical AuNPs seed-mediated growth method by altering the amount of AuNPs in reaction system had been synthesized. The UV-vis spectrum of synthesized SUL-AuNPs displayed a shift from 550 nm to 650 nm compared to spherical AuNPs. FRET method was applied for the detection of GSH in hepatocytes and cell extracts using rhodamine B (RB) functionalized SUL-AuNPs, among the synthesized three different diameters SUL-AuNPs, 100nm RB-SUL-AuNPs displayed highest sensitivity for GSH detection. What's more, all synthesized SUL-AuNPs turned out to be membrane-permeable, and displayed ignorable cytotoxicity, which make SUL-AuNPs promising cellular thiols detection probes. In particular, it should be noted that the application of RB functionalized SUL-AuNPs exemplify ongoing efforts in design and utility of multifunctional nanoplatforms of SUL-AuNPs.
VL  - 10
IS  - 2
ER  -

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Author Information

Ting Lei Zhao

College of Chemistry and Chemical Engineering, Linyi University, Linyi, China
Zhe Zhang

College of Chemistry and Chemical Engineering, Jinan University, Jinan, China
Dan Li

College of Chemistry and Chemical Engineering, Linyi University, Linyi, China
Yanxialei Jiang

College of Chemistry and Chemical Engineering, Linyi University, Linyi, China

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APA Style

Ting Lei Zhao, Zhe Zhang, Dan Li, Yanxialei Jiang. (2022). Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. International Journal of Biomedical Science and Engineering, 10(2), 54-60. https://doi.org/10.11648/j.ijbse.20221002.14

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ACS Style

Ting Lei Zhao; Zhe Zhang; Dan Li; Yanxialei Jiang. Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. Int. J. Biomed. Sci. Eng. 2022, 10(2), 54-60. doi: 10.11648/j.ijbse.20221002.14

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AMA Style

Ting Lei Zhao, Zhe Zhang, Dan Li, Yanxialei Jiang. Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. Int J Biomed Sci Eng. 2022;10(2):54-60. doi: 10.11648/j.ijbse.20221002.14

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@article{10.11648/j.ijbse.20221002.14,
  author = {Ting Lei Zhao and Zhe Zhang and Dan Li and Yanxialei Jiang},
  title = {Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {10},
  number = {2},
  pages = {54-60},
  doi = {10.11648/j.ijbse.20221002.14},
  url = {https://doi.org/10.11648/j.ijbse.20221002.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20221002.14},
  abstract = {Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination of physical and optical properties. Sea urchin-like gold nanoparticles (SUL-AuNPs) are well characterized multi-thorn gold nanostructures which possess at least four gold nanothorns on spherical gold surface, mimicking the morphology of sea urchin. Compared to spherical AuNPs, SUL-AuNPs showed a wide variety of light absorption and scattering properties, and the Surface-enhanced Raman Scattering (SERS) properties of SUL-AuNPs were also widely studied dependent on their surface morphology. Herein, three different diameters of SUL-AuNPs based on spherical AuNPs seed-mediated growth method by altering the amount of AuNPs in reaction system had been synthesized. The UV-vis spectrum of synthesized SUL-AuNPs displayed a shift from 550 nm to 650 nm compared to spherical AuNPs. FRET method was applied for the detection of GSH in hepatocytes and cell extracts using rhodamine B (RB) functionalized SUL-AuNPs, among the synthesized three different diameters SUL-AuNPs, 100nm RB-SUL-AuNPs displayed highest sensitivity for GSH detection. What's more, all synthesized SUL-AuNPs turned out to be membrane-permeable, and displayed ignorable cytotoxicity, which make SUL-AuNPs promising cellular thiols detection probes. In particular, it should be noted that the application of RB functionalized SUL-AuNPs exemplify ongoing efforts in design and utility of multifunctional nanoplatforms of SUL-AuNPs.},
 year = {2022}
}

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TY  - JOUR
T1  - Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles
AU  - Ting Lei Zhao
AU  - Zhe Zhang
AU  - Dan Li
AU  - Yanxialei Jiang
Y1  - 2022/06/27
PY  - 2022
N1  - https://doi.org/10.11648/j.ijbse.20221002.14
DO  - 10.11648/j.ijbse.20221002.14
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 54
EP  - 60
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20221002.14
AB  - Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination of physical and optical properties. Sea urchin-like gold nanoparticles (SUL-AuNPs) are well characterized multi-thorn gold nanostructures which possess at least four gold nanothorns on spherical gold surface, mimicking the morphology of sea urchin. Compared to spherical AuNPs, SUL-AuNPs showed a wide variety of light absorption and scattering properties, and the Surface-enhanced Raman Scattering (SERS) properties of SUL-AuNPs were also widely studied dependent on their surface morphology. Herein, three different diameters of SUL-AuNPs based on spherical AuNPs seed-mediated growth method by altering the amount of AuNPs in reaction system had been synthesized. The UV-vis spectrum of synthesized SUL-AuNPs displayed a shift from 550 nm to 650 nm compared to spherical AuNPs. FRET method was applied for the detection of GSH in hepatocytes and cell extracts using rhodamine B (RB) functionalized SUL-AuNPs, among the synthesized three different diameters SUL-AuNPs, 100nm RB-SUL-AuNPs displayed highest sensitivity for GSH detection. What's more, all synthesized SUL-AuNPs turned out to be membrane-permeable, and displayed ignorable cytotoxicity, which make SUL-AuNPs promising cellular thiols detection probes. In particular, it should be noted that the application of RB functionalized SUL-AuNPs exemplify ongoing efforts in design and utility of multifunctional nanoplatforms of SUL-AuNPs.
VL  - 10
IS  - 2
ER  -

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