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Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes
Tadese Setegn,
Tesfu Fekensa,
Emiru Siyoum,
Solomon Mekonnen
Issue:
Volume 4, Issue 3, June 2016
Pages:
13-21
Received:
6 May 2016
Accepted:
20 May 2016
Published:
30 June 2016
Abstract: Laboratory investigation was conducted to know the effect of irradiation (sterility) on reproductive performance of females G. pallidipes mated with sterile and fertile males under temperature of 23-25°C and 75-80% relative humidity. Completely randomized designs with three replications were followed throughout the experiments. The irradiation effects on male G. pallidipes at different doses of irradiation were checked using, 60Gy, 80Gy, 100Gy and 120Gy. Results showed that at highest doses the sterility effects on male Glossina pallidipes were significantly lower than lowest doses (P<0.05) on pupae production and emergence of adults. On the other hand, the highest dose (120Gy) caused abortion of egg/larvae significantly higher than the lower dose (60Gy) (P<0.05). In other investigation which was aimed to assessing the competitiveness of sterile malestofertile males in a cage at ratios of 9 sterile: 1 fertile males, 7 sterile to 3 fertile males, 5 sterile to 5 fertile males and 3 sterile to 7 fertile males, the results showed that the ratio with the highest number of sterile (9:1) in the cage caused significantly low number of pupae and number of adults emerged from pupae than those with lower number of sterile males (3:7) (P<0.05). Similarly, the ratio with the highest sterile males (9:1) resulted in significantly higher number of egg/larvae abortion than those with lower number of sterile males (3:7) ratio (P<0.05). In the present study, survival of male G. pallidipes irradiated with 120Gy three days after emergence from pupae was significantly higher than males irradiatedone and ten days after emergence from pupae (P<0.05). This may indicate that irradiated males may need to rest up to 7 or 10 days before released to the field. The importance of sterilization using appropriate dose, the appropriate ratio of sterile to fertile males and the time at which sterility was done efficiently all are relevant.
Abstract: Laboratory investigation was conducted to know the effect of irradiation (sterility) on reproductive performance of females G. pallidipes mated with sterile and fertile males under temperature of 23-25°C and 75-80% relative humidity. Completely randomized designs with three replications were followed throughout the experiments. The irradiation eff...
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Erythrocyte Morphological Characteristics Based on Microscope Images System
Issue:
Volume 4, Issue 3, June 2016
Pages:
22-27
Received:
19 September 2016
Published:
27 September 2016
Abstract: Microscope images analysis of erythrocyte (red blood cells, RBCs) was a widely used method for medical purpose. Usually manual measuring and analysis of the images were subject to time-consuming, errors and unstability of results. The images analysis method had been combined with computer image processing techniques in this research. A measuring and analysis system for microscope images(MIAS) of RBCs was developed, which could recognize RBCs in images and measure cells mophometric parameters. Normal human RBCs were compared with ones under high glucose. The results indicated RBCs sizes parameters such as areas, perimeters, major axis lengths, minor axis lengths, elongations, roundnesses and Feret diameters have difference between normal and high glucose conditions. RBCs normal disk shapes changed into acanthocyte and stomatocyte under higher glucose conditions. This fast and precise method for measuring RBCs morphometric parameters contributed to pathogenesis of diabetic nephropathy(DN) research.
Abstract: Microscope images analysis of erythrocyte (red blood cells, RBCs) was a widely used method for medical purpose. Usually manual measuring and analysis of the images were subject to time-consuming, errors and unstability of results. The images analysis method had been combined with computer image processing techniques in this research. A measuring an...
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Preparation on Chitosan Derivative Nanoparticles as Prolonged Releasing Drug Carrier
Issue:
Volume 4, Issue 3, June 2016
Pages:
28-33
Received:
19 September 2016
Published:
27 September 2016
Abstract: Ricinoleic acid grafted amphiphilic carboxymethy chitosan (CMC-g-RA) was synthetised as a carrier to load botanical drug rotenone (Rot). Then Rot/CMC-g-RA water dispersion in nanoscale was prepared, whose shape, zeta potential, loading efficiency and outdoor stability were characterized accordingly. The results indicated that the sizes, polydispersity index, zeta potential of Rot/CMC-g-RA particles were affected by concentrations of this water dispersion. When the ratio of carrier to drug ascended, the water dispersion had monodisperse nanoparticle sizes with negative charge on nanoparticle surface. And the water dispersion restrained Rot degradation in natural environment with higher loading efficiency.
Abstract: Ricinoleic acid grafted amphiphilic carboxymethy chitosan (CMC-g-RA) was synthetised as a carrier to load botanical drug rotenone (Rot). Then Rot/CMC-g-RA water dispersion in nanoscale was prepared, whose shape, zeta potential, loading efficiency and outdoor stability were characterized accordingly. The results indicated that the sizes, polydispers...
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