Optimizing Different Conditions to Develop a Single Stranded Phage-Lambda DNA (λDNA) Tightrope
Issue:
Volume 7, Issue 3, September 2019
Pages:
61-67
Received:
14 August 2019
Accepted:
9 September 2019
Published:
24 September 2019
Abstract: λDNA tightrope can be referred to as λDNA is suspended between two beads above the surface of the flow cell which allows the λDNA to be viewed extended rather than in collapse form. It is use for DNA studies such DNA repairs, but when using the normal protocol (at a velocity of 300 µl/minutes, beads size of 5 µm and concentration of 500.0 µg/ml of 1.0 λDNA) it results to multiple tightropes forming on the beads which interference with a many studies. In this study, different conditions were optimized to develop a single stranded λDNA tight rope by taking into consideration the following conditions, velocity (100 to 600), beads size (3 µm and 5µm), concentration (500.0 µg/ml, 375.0 µg/ml and 250.0 µg/ml) and time. To form the tightrope, beads were added into water in an eppendorf tube, centrifuged and sonicated before putting into the flow cell. The flow cell, syringe pump and perfusion tube were all fixed together, where ABC buffer and λDNA were put into the perfusion tube and set according to protocol to make the tightropes. YOYO-1 dye was used to stain the λDNA before using atomic force microscopy to image the tightropes, the same procedure was repeated for the different conditions and all dispense readings of the flow rate were taken at not less than 3.0 µl/minutes. λDNA on the surface of the flow cell was observed at the velocity of 100 and 200 µl/minutes for all the conditions. The optimized result was at velocity of 300µl/ml, concentration 375.0 µg/ml of 1.0 μλDNA and beads size of 3 µl which lasted for 15 minutes which gave the best result of single stranded λDNA tightrope compared to all the conditions and the normal protocol.
Abstract: λDNA tightrope can be referred to as λDNA is suspended between two beads above the surface of the flow cell which allows the λDNA to be viewed extended rather than in collapse form. It is use for DNA studies such DNA repairs, but when using the normal protocol (at a velocity of 300 µl/minutes, beads size of 5 µm and concentration of 500.0 µg/ml of ...
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Assessment of Knowledge About Effects of Coconut Oil on Human Health Among Students of Hail University
Yomna Ali Moustafa Marzok Elkhateeb,
Salma Bashir Alrashidi,
Ebtesam Hdeeb Alshammary
Issue:
Volume 7, Issue 3, September 2019
Pages:
68-74
Received:
15 August 2019
Accepted:
9 September 2019
Published:
25 September 2019
Abstract: For several years, Consumers were made to believe that coconut oil is deleterious to health as it would block the arteries and cause heart disease. In recent years Coconut oil is receiving attention as a Functional food oil and its consumption has dramatically risen. Coconut oil has been claimed to have beneficial effects on health. There are rarely published studies from Saudi Arabia exploring the awareness about benefits or risks of coconut oil. The objective of the present work is to evaluate the knowledge of students of Hail University about the effects of coconut oil on health with a comparison between females (scientific and Literary College) and males (scientific and Literary College). Also, this report aims to review the available literature about benefits and risks of coconut oil consumption and highlight its positive effects on health. Results of the present study was concluded that students of University have a moderate knowledge about effect of coconut oil on human health but the knowledge of female students more than male students. Comparison between students of scientific College and Literary College in level of knowledge about healthy importance of coconut oil show a none significant difference in all questions. The present work concluded that coconut oil is the best oil for health and reported the need to increase the knowledge of students about beneficial of coconut oil by distributing booklets or by health education program to highlight its positive effects on health and correct their thinking that coconut oil is harmful.
Abstract: For several years, Consumers were made to believe that coconut oil is deleterious to health as it would block the arteries and cause heart disease. In recent years Coconut oil is receiving attention as a Functional food oil and its consumption has dramatically risen. Coconut oil has been claimed to have beneficial effects on health. There are rarel...
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Cavitated Lesion in a Patient Infected by the Human Immunodeficiency Virus (HIV)
Aceituno Cano Alexandra Maria,
Gaquez Aguilera Elena,
Galindo Flores Maria Fernanda,
Vogt Sanchez Esteban Alessandro,
Collado Romacho Antonio Ramon
Issue:
Volume 7, Issue 3, September 2019
Pages:
75-78
Received:
8 August 2019
Accepted:
5 September 2019
Published:
2 October 2019
Abstract: It is known that the progressive deterioration of the immune system in the human immunodeficiency syndrome (AIDS), either cellular or humoral level and the obligated role of exchange of the respiratory system with the external environment, makes the incidence of inflammatory, tumoral, and infectious lung processes high in patients infected with the human immunodeficiency virus (HIV). There are several microorganisms that can cause cavitated lung lesions in a HIV patient as Pneumocystis carinii, Mycobacterium tuberculosis or Staphylococcus aureus, among others. Rhodoccoccus equi (R. Equi) is, predominantly, an opportunistic pathogen who can also cause disease in humans, especially in immunocompromised patients. This report describe a case of Rhodococcus equi lung infection in a HIV patient, which highlights the importance of the therapheutic adherence and the epidemiological enviroment in this kind of patients.
Abstract: It is known that the progressive deterioration of the immune system in the human immunodeficiency syndrome (AIDS), either cellular or humoral level and the obligated role of exchange of the respiratory system with the external environment, makes the incidence of inflammatory, tumoral, and infectious lung processes high in patients infected with the...
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